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2006年02月01日
ニキビ菌に関する報告書
ただいま、タイにおきまして臨床実験中です。
P.acnes total count on TiO2 sprayMethod 1
- prepare BHA agar in DI water and sterilize with autoclave for 20 min.
- add glucose in BHA to have final concentration 1%
- pour agar base in each plate to be 15 mL, leave until it get harden
- spread sample (spray 0.5 or 1.0 mL) per plate
- prepare P.acnes at MCF = 0.5 (number colony = 108 CFU/mL)
- prepare P.acnes at varying dilution 10-1, 10-2 , 10-3 , 10-4 , 10-5 , 10-6
- mix each dilution of P.acnes 100 uL of 10-5 , 10-6 with 4.5 mL BHA broth
- pour broth on the plate and leave until it become solid
- incubate at anaerobic condition at 37 °C in anaerobic jar for 3 days
Result
| formulation | Number of colony (Dilution 10-5) | Number of colony (Dilution 10-6) | ||||
| R1 | R2 | R3 | R1 | R2 | R3 | |
| Spray (0.5 mL) | - | 1.7×107 | 3.0×106 | - | 2.0×107 | * |
| Spray (1.0 mL) | 4.3×106 | * | 2.3×106 | 2.7×107 | 3.3×106 | * |
| Positive control | 2.0×106 | * | * | * | * | * |
| Negative control -UV | 8.7×107 | 5.4×107 | 1.9×107 | 4.6×107 | 6.0×107 | 3.7×107 |
* = no P.acnes growth
Positive control = clinda M ®
Note
- pray (for both 0.5 and 1 mL) can reduce the growth of P.acnes at all dilutions although the inhibition ability is lower than positive control .
- Higher amount of spray (1 mL) can inhibit the growth of microbe better than lower volume.
P.acnes total count on TiO2 spray
Method 2
- prepare BHA agar in DI water and sterilize with autoclace for 20 min.
- add glucose in BHA to have final concentration 1%
- pour agar base in each plate to be 15 mL, leave until it get harden
- prepare BHA seed agar 4 mL and sample (spray 1.0 mL) per plate
- pour BHA seed agar on top of base agar, leave until harden
- prepare P.acnes at MCF = 0.5 (number colony = 108 CFU/mL)
- prepare P.acnes at varying dilution 10-1, 10-2 , 10-3 , 10-4 , 10-5 , 10-6
- spread dilution of P.acnes 100 uL of 10-4 , 10-5 , 10-6 dilution
- incubate at anaerobic condition at 37 °C in anaerobic jar for 3 days
Result
| formulation | Number of colony (Dilution 10-4) | Number of colony (Dilution 10-5) | Number of colony (Dilution 10-6) | ||||||
| R1 | R2 | R3 | R1 | R2 | R3 | R1 | R2 | R3 | |
| Spray (1.0 mL) | 2×107 | 1.2×107 | 1.8×107 | 9.5×107 | 2.5×107 | 3.7×107 | 5.0×107 | 6.0×107 | 3.3×107 |
| Positive control | * | * | * | * | * | * | * | * | * |
| Negative control | >3×107 | >3×107 | >3×107 | >3×108 | 9.1×107 | 6.5×107 | 6×108 | 4.9×108 | 7.7×107 |
* = no P.acnes growth
Positive control = clinda M ®
Note
- Spary can reduce the growth of P.acnes as compared to negative control without any treatment. However the inhibition ability towards microbe is still lower than positive control (clindamycin)
Dr.Uracha
投稿者 Webmaster : 2006年02月01日 14:24
